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Preclinical Evaluation of Photoacoustic Imaging as a Novel Noninvasive Approach to Detect an Orthopaedic Implant Infection.

  • Author(s): Wang, Yu
  • Thompson, John M
  • Ashbaugh, Alyssa G
  • Khodakivskyi, Pavlo
  • Budin, Ghyslain
  • Sinisi, Riccardo
  • Heinmiller, Andrew
  • van Oosten, Marleen
  • van Dijl, Jan Maarten
  • van Dam, Gooitzen M
  • Francis, Kevin P
  • Bernthal, Nicholas M
  • Dubikovskaya, Elena A
  • Miller, Lloyd S
  • et al.
Abstract

Introduction

Diagnosing prosthetic joint infection (PJI) poses significant challenges, and current modalities are fraught with low sensitivity and/or potential morbidity. Photoacoustic imaging (PAI) is a novel ultrasound-based modality with potential for diagnosing PJI safely and noninvasively.

Materials

In an established preclinical mouse model of bioluminescent Staphylococcus aureus PJI, fluorescent indocyanine green (ICG) was conjugated to β-cyclodextrin (CDX-ICG) or teicoplanin (Teic-ICG) and injected intravenously for 1 week postoperatively. Daily fluorescent imaging and PAI were used to localize and quantify tracer signals. Results were analyzed using 2-way analysis of variance.

Results

Fluorescence clearly localized to the site of infection and was significantly higher with Teic-ICG compared with CDX-ICG (P = 0.046) and ICG alone (P = 0.0087). With PAI, the photoacoustic signal per volumetric analysis was substantially higher and better visualized with Teic-ICG compared with CDX-ICG and ICG alone, and colocalized well with bioluminescence and fluorescence imaging.

Conclusion

Photoacoustic imaging successfully localized PJI in this proof-of-concept study and demonstrates potential for clinical translation in orthopaedics.

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