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Open Access Publications from the University of California

Methods to Study the Atypical Roles of DNA Repair and SMC Proteins in Gene Silencing.

  • Author(s): Peterson, Misty R
  • Hamdani, Omar
  • Kamakaka, Rohinton T
  • et al.

Silenced heterochromatin influences all nuclear processes including chromosome structure, nuclear organization, transcription, replication, and repair. Proteins that mediate silencing affect all of these nuclear processes. Similarly proteins involved in replication, repair, and chromosome structure play a role in the formation and maintenance of silenced heterochromatin. In this chapter we describe a handful of simple tools and methods that can be used to study the atypical role of proteins in gene silencing.

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