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Floating droplet array: An ultrahigh-throughput device for droplet trapping, real-time analysis and recovery

  • Author(s): Labanieh, L
  • Nguyen, TN
  • Zhao, W
  • Kang, DK
  • et al.

Published Web Location

https://doi.org/10.3390/mi6101431Creative Commons Attribution 4.0 International Public License
Abstract

© 2015 by the authors. We describe the design, fabrication and use of a dual-layered microfluidic device for ultrahigh-throughput droplet trapping, analysis, and recovery using droplet buoyancy. To demonstrate the utility of this device for digital quantification of analytes, we quantify the number of droplets, which contain a β-galactosidase-conjugated bead among more than 100,000 immobilized droplets. In addition, we demonstrate that this device can be used for droplet clustering and real-time analysis by clustering several droplets together into microwells and monitoring diffusion of fluorescein, a product of the enzymatic reaction of β-galactosidase and its fluorogenic substrate FDG, between droplets.

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