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Single step isolation and activation of primary CD3+T lymphocytes using alcohol-dispersed electrospun magnetic nanofibers

  • Author(s): Kim, K
  • An, HJ
  • Jun, SH
  • Kim, TJ
  • Lim, SA
  • Park, G
  • Na, HB
  • Park, YI
  • Hyeon, T
  • Yee, C
  • Bluestone, JA
  • Kim, J
  • Lee, KM
  • et al.

Published Web Location

https://doi.org/10.1021/nl301388d
Abstract

Electrospun polymer nanofibers with entrapped magnetic nanoparticles (magnetic NP-NF) represent a novel scaffold substrate that can be functionalized for single-step isolation and activation of specific lymphocyte subsets. Using a surface-embedded T cell receptor ligand/trigger (anti-CD3 monoclonal antibody), we demonstrate, as proof of principle, the use of magnetic NP-NF to specifically isolate, enrich, and activate CD3+T cells from a heterogeneous cell mixture, leading to preferential expansion of CD8+CD3+T cells. The large surface area, adjustable antibody density, and embedded paramagnetic properties of the NP-NF permitted enhanced activation and expansion; its use represents a strategy that is amenable to an efficient selection process for adoptive cellular therapy as well as for the isolation of other cellular subsets for downstream translational applications. © 2012 American Chemical Society.

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