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Single step isolation and activation of primary CD3+ T lymphocytes using alcohol-dispersed electrospun magnetic nanofibers.

  • Author(s): Kim, Kwanghee;
  • An, Hyo Jin;
  • Jun, Seung-Hyun;
  • Kim, Tae-Jin;
  • Lim, Seon Ah;
  • Park, Gayoung;
  • Na, Hyon Bin;
  • Park, Yong Il;
  • Hyeon, Taeghwan;
  • Yee, Cassian;
  • Bluestone, Jeffrey A;
  • Kim, Jungbae;
  • Lee, Kyung-Mi
  • et al.

Published Web Location

https://doi.org/10.1021/nl301388d
Abstract

Electrospun polymer nanofibers with entrapped magnetic nanoparticles (magnetic NP-NF) represent a novel scaffold substrate that can be functionalized for single-step isolation and activation of specific lymphocyte subsets. Using a surface-embedded T cell receptor ligand/trigger (anti-CD3 monoclonal antibody), we demonstrate, as proof of principle, the use of magnetic NP-NF to specifically isolate, enrich, and activate CD3(+) T cells from a heterogeneous cell mixture, leading to preferential expansion of CD8(+)CD3(+) T cells. The large surface area, adjustable antibody density, and embedded paramagnetic properties of the NP-NF permitted enhanced activation and expansion; its use represents a strategy that is amenable to an efficient selection process for adoptive cellular therapy as well as for the isolation of other cellular subsets for downstream translational applications.

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