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Open Access Publications from the University of California

A Hypothalamic Switch for REM and Non-REM Sleep

  • Author(s): Chen, Kai-Siang
  • Advisor(s): Dan, Yang
  • et al.
No data is associated with this publication.

Rapid eye movement (REM) and non-REM (NREM) sleep are controlled by specific neuronal circuits. In this thesis, I show that galanin-expressing GABAergic neurons in the dorsomedial hypothalamus (DMH) comprise separate subpopulations with opposing effects on REM versus NREM sleep. Microendoscopic calcium imaging revealed diverse sleep-wake activity of DMH GABAergic neurons, but the galanin-expressing subset falls into two distinct groups, either selectively activated (REM-on) or suppressed (REM-off) during REM sleep. Retrogradely labeled, preoptic area (POA)-projecting galaninergic neurons are REM-off, whereas the raphe pallidus (RPA)-projecting neurons are primarily REM-on. Bidirectional optogenetic manipulations showed that the POA projectors promote NREM sleep and suppress REM sleep, while the RPA projectors have the opposite effects. Thus, REM/NREM switch is regulated antagonistically by galaninergic neurons with intermingled cell bodies but distinct axon projections.

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This item is under embargo until March 9, 2022.