Skip to main content
eScholarship
Open Access Publications from the University of California

UCSF

UC San Francisco Electronic Theses and Dissertations bannerUCSF

The pursuit of membrane protein structures and the Malarial aquaglyceroporin PfAQP

Abstract

When I began my thesis work, structures of membrane proteins were few and far between. It was clear that the field was poised for a growth period and offered myriad opportunities for pursuing targets involved in important and interesting biology. It was also clear that there was a dire need to develop new methods and protocols to express, purify and crystallize membrane proteins. Described here are my efforts to contribute to this growth period of membrane protein structural biology. This includes establishing cell culture facilities and protocols for the production of milligram quantities of mammalian membrane proteins, the solving of the structure of the Malarial aquaglyceroporin PfAQP and a carefully designed expression screen that has resulted so far in the crystallization of four proteins. The structure of one of theses proteins, CcmG, has been solved.

Main Content
For improved accessibility of PDF content, download the file to your device.
Current View