Lawrence Berkeley National Laboratory

A draft sequence of the genome of the moderate halophile chromohalobacter salexigens (formerly halomonas elongata) dsm3043 (fig. 1) has been determined to an 8x Coverage by the joint genome institute (jgi) of the us department of energy. From this Sequence, 3370 predicted protein-coding genes were identified and provisionally Annotated by computer analysis at the jgi. The draft sequence and annotation are Available at http://genome.jgi-psf.org/microbial/index.html. In this chapter, we present a Brief overview of insights we gained into metabolism in c. Salexigens from the sequence. In view of the close phylogenetic relationship of c. Salexigens to the pseudomonaceae And other ?-proteobacteria (see below), we used known enzyme sequences from Pseudomonaceae, acinetobacter, escherichia coli, other enterobacteriaceae, and other Bacteria (in this order of priority) as query sequences for orthologs in c. Salexigens. Any Match with a probability ("expect value" in tbastn; Http://www.ncbi.nlm.nih.gov/blast) ? e-20 was considered to be highly significant, Values between e-5 and e-20 to be marginal, and ? e-5 to be not significant. The usual Disclaimers about inferences from genomic sequence are in order: that all of the Annotations that we are assigning are provisional and await confirmation by biochemical Or functional genetic analyses, and that the predictions of the functions of the c. Salexigens gene products are only as valid as the accuracy of the assignment of the Function of matching proteins in other organisms.