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Combining multivariate analysis and monosaccharide composition modeling to identify plant cell wall variations by Fourier Transform Near Infrared spectroscopy.

  • Author(s): Smith-Moritz, Andreia M
  • Chern, Mawsheng
  • Lao, Jeemeng
  • Sze-To, Wing Hoi
  • Heazlewood, Joshua L
  • Ronald, Pamela C
  • Vega-Sánchez, Miguel E
  • et al.
Abstract

We outline a high throughput procedure that improves outlier detection in cell wall screens using FT-NIR spectroscopy of plant leaves. The improvement relies on generating a calibration set from a subset of a mutant population by taking advantage of the Mahalanobis distance outlier scheme to construct a monosaccharide range predictive model using PLS regression. This model was then used to identify specific monosaccharide outliers from the mutant population.

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