Imaging in turbid media: a transmission detector gives 2-3 order of magnitude enhanced sensitivity compared to epi-detection schemes
- Author(s): Dvornikov, A
- Gratton, E
- et al.
Published Web Locationhttps://doi.org/10.1364/BOE.7.003747
Imaging depth in turbid media by two-photon fluorescence microscopy depends on the ability of the optical system to detect weak fluorescence signals. We have shown that use of a wide area detector in transmission geometry allows increasing imaging depth in turbid media due to efficient photon collection. Compared to the conventional epi-detection scheme used in most commercial microscopes, the transmission detector was found to be 2-3 orders of magnitude more sensitive when used for in depth imaging in scattering samples simulating brain optical properties.
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