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Software and methods for oligonucleotide and cDNA array data analysis.

  • Author(s): Zapala, Matthew A
  • Lockhart, Daniel J
  • Pankratz, Daniel G
  • Garcia, Anthony J
  • Barlow, Carrolee
  • Lockhart, David J
  • et al.
Abstract

Two HTML-based programs were developed to analyze and filter gene-expression data: 'Bullfrog' for Affymetrix oligonucleotide arrays and 'Spot' for custom cDNA arrays. The programs provide intuitive data-filtering tools through an easy-to-use interface. A background subtraction and normalization program for cDNA arrays was also built that provides an informative summary report with data-quality assessments. These programs are freeware to aid in the analysis of gene-expression results and facilitate the search for genes responsible for interesting biological processes and phenotypes.

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