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3D Nanometer Images of Biological Fibers by Directed Motion of Gold Nanoparticles

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https://doi.org/10.1021/nl2022042Creative Commons 'BY' version 4.0 license
Abstract

Using near-infrared femtosecond pulses, we move single gold nanoparticles (AuNPs) along biological fibers, such as collagen and actin filaments. While the AuNP is sliding on the fiber, its trajectory is measured in three dimensions (3D) with nanometer resolution providing a high-resolution image of the fiber. Here, we systematically moved a single AuNP along nanometer-size collagen fibers and actin filament inside chinese hamster ovary K1 living cells, mapping their 3D topography with high fidelity.

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