Skip to main content
eScholarship
Open Access Publications from the University of California

Mechanogenetics for the remote and noninvasive control of cancer immunotherapy.

  • Author(s): Pan, Yijia
  • Yoon, Sangpil
  • Sun, Jie
  • Huang, Ziliang
  • Lee, Changyang
  • Allen, Molly
  • Wu, Yiqian
  • Chang, Ya-Ju
  • Sadelain, Michel
  • Shung, K Kirk
  • Chien, Shu
  • Wang, Yingxiao
  • et al.

Published Web Location

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5798350/
No data is associated with this publication.
Abstract

While cell-based immunotherapy, especially chimeric antigen receptor (CAR)-expressing T cells, is becoming a paradigm-shifting therapeutic approach for cancer treatment, there is a lack of general methods to remotely and noninvasively regulate genetics in live mammalian cells and animals for cancer immunotherapy within confined local tissue space. To address this limitation, we have identified a mechanically sensitive Piezo1 ion channel (mechanosensor) that is activatable by ultrasound stimulation and integrated it with engineered genetic circuits (genetic transducer) in live HEK293T cells to convert the ultrasound-activated Piezo1 into transcriptional activities. We have further engineered the Jurkat T-cell line and primary T cells (peripheral blood mononuclear cells) to remotely sense the ultrasound wave and transduce it into transcriptional activation for the CAR expression to recognize and eradicate target tumor cells. This approach is modular and can be extended for remote-controlled activation of different cell types with high spatiotemporal precision for therapeutic applications.

Many UC-authored scholarly publications are freely available on this site because of the UC's open access policies. Let us know how this access is important for you.

Item not freely available? Link broken?
Report a problem accessing this item