Toohey-Kurth, Kathy L; Mulrooney, Donna M; Hinkley, Susanne; Killian, Mary Lea; Pedersen, Janice C; Bounpheng, Mangkey A; Pogranichniy, Roman; Bolin, Steve; Maes, Roger; Tallmadge, Rebecca L; Goodman, Laura B; Crossley, Beate M

doi:10.1177/1040638720962076

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Best practices for performance of real-time PCR assays in veterinary diagnostic laboratories

2020

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https://doi.org/10.1177/1040638720962076

Abstract

The exquisite sensitivity of in vitro amplification assays such as real-time polymerase chain reaction (rtPCR) requires the establishment of thorough and robust laboratory practices. To this end, an American Association of Veterinary Laboratory Diagnosticians (AAVLD) committee of subject matter experts was convened to develop a set of best practices for performance of nucleic acid amplification assays. Consensus advice for the performance of preanalytical, analytical, and postanalytical steps is presented here, along with a review of supporting literature.

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Best practices for performance of real-time PCR assays in veterinary diagnostic laboratories

Published Web Location