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Open Access Publications from the University of California

AQU2: Detection and Identification of Aquatic Microorganisms

  • Author(s): Beth Stauffer
  • Mrinal Mahapatro
  • Chongwu Zhou
  • Ari Requicha
  • Alex Lee
  • Chao Li
  • Stefanie Moorthi
  • Dave Caron
  • et al.
Abstract

With an increase in blooms of harmful algae on both national and global levels, it is becoming ever more important to be able to detect these organisms at high spatial and temporal resolutions. It is also imperative that methods of detection are able to identify target cells at low concentrations. To this end we present a number of immunologically-based techniques for detecting cells of Aureococcus anophagefferens, the cause of ’�¡�ÈBrown Tides’�¡�É off the Mid-Atlantic coast. These techniques include flow cytometry, atomic force microscopy, and nanowire- and nanotube-based sensing. We also detail experiments carried out in a laboratory testbed demonstrating ecologically significant behavior patterns in two harmful algal species responsible for ’�¡�ÆBrown’�¡�Ç and ’�¡�ÆRed’�¡�Ç tides on the east and west coast, respectively.

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