Effect of freezing on the relative escape force of sperm as measured by a laser optical trap*†*Presented at the 8th World Congress of In Vitro Fertilization and Alternate Assisted Reproduction, Kyoto, Japan, September 11 to 15, 1993.†Supported by grant RR-01192 from the National Institutes of Health, Bethesda, Maryland; by grant 000-14-91-C-0134 from the Office of Naval Research, Arlington, Virginia; and by grant DE-FG03-91 ER 61227 from the Department of Energy, Washington, D.C.
Published Web Location
https://doi.org/10.1016/s0015-0282(16)57316-6
Abstract
OBJECTIVE: To determine the possible effect of freezing on sperm escape force as measured by laser trap. DESIGN: Controlled clinical study. SETTING: Normal volunteers, academic setting. PATIENTS: Normal, healthy volunteers. INTERVENTION: Sperm selection, freezing and thawing. Measurement of relative (sperm) escape force before and after freezing. MAIN OUTCOME MEASURE: Comparison of escape force as a measurement of freezing effects. RESULTS: Wide individual variations noted, with some individuals showing significant increases or decreases. The relative escape force in fresh (76.1 +/- 31.1 mW) and in frozen-thawed samples (75.6 +/- 40.0 mW) were similar. CONCLUSION: Freezing does not affect the relative escape force of normal sperm. Any possible detrimental effect of freezing on sperm may be related to other sperm functions.
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