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Isolating Arabidopsis mutants affecting auxin-mediated development

  • Author(s): Du, Jean Minzhi
  • et al.

Auxin is a plant hormone that plays essential roles in all aspects of growth and development. Plants have multiple pathways to synthesize auxin. The 11 YUCCA genes encode for FMO-like enzymes and catalyze the conversion of tryptamine to N-hydroxyl-tryptamine; they participate in tryptophan-dependent auxin biosynthesis branch. Molecular analysis of auxin mechanisms is difficult because genes involved in auxin biosynthesis, transport, and signaling oftentimes belong to gene families. Here I conduct EMS forward genetic screening in the partially auxin deficient yuc1-/-yuc4-/+ background. I have isolated enhancer and suppressor mutants and attempted to identify the corresponding genes. In addition, a weak allele of the auxin transport gene PINOID - pid336, was cloned. Using pid336, I initiate another genetic screening employing T- DNA activation tagging method, in the sensitized yuc1-/- pid336-/- background. In total, I have screened more than two thousand lines, isolated several mutants, and have attempted to identify the genes involved. The identification and characterization of genes from my screening help to elucidate the molecular mechanisms of auxin biosynthesis and auxin signaling

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