Lawrence Berkeley National Laboratory

The Joint Genome Institute Production Genomics Facility (PGF) has produced over 2.75 billion bases of draft paired-end sequencing since January 1, 2001. Our sequencing methodologies incorporate two types of DNA template generation: inoculation/SPRI purification and Rolling Circle Amplification (RCA). In order to manage the flow of samples through these processes, a robust database tracking system was developed using ORACLE. Data input and reporting for the workflow has been produced using a combination of commercial database development software and in-house programs. These include ORACLE s WebDB and CGI Perl programming. By leveraging the rapid report and form development cycle using WebDB and augmenting this with the flexibility of in-house programming, we have efficiently deployed a critical laboratory information management system for our data tracking.