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Production workflow tracking and QC analysis

  • Author(s): Kimball, Heather
  • Trong, Stephan
  • Kobayashi, Art
  • Pitluck, Sam
  • Lou, Yunian
  • Nolan, Matt
  • et al.
Abstract

The Joint Genome Institute Production Genomics Facility (PGF) has produced over 2.75 billion bases of draft paired-end sequencing since January 1, 2001. Our sequencing methodologies incorporate two types of DNA template generation: inoculation/SPRI purification and Rolling Circle Amplification (RCA). In order to manage the flow of samples through these processes, a robust database tracking system was developed using ORACLE. Data input and reporting for the workflow has been produced using a combination of commercial database development software and in-house programs. These include ORACLE s WebDB and CGI Perl programming. By leveraging the rapid report and form development cycle using WebDB and augmenting this with the flexibility of in-house programming, we have efficiently deployed a critical laboratory information management system for our data tracking.

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