A fast and selective near-infrared fluorescent sensor for multicolor imaging of biological nitroxyl (HNO).
- Author(s): Wrobel, Alexandra T
- Johnstone, Timothy C
- Deliz Liang, Alexandria
- Lippard, Stephen J
- Rivera-Fuentes, Pablo
- et al.
Published Web Locationhttps://doi.org/10.1021/ja500315x
The first near-infrared fluorescent turn-on sensor for the detection of nitroxyl (HNO), the one-electron reduced form of nitric oxide (NO), is reported. The new copper-based probe, CuDHX1, contains a dihydroxanthene (DHX) fluorophore and a cyclam derivative as a Cu(II) binding site. Upon reaction with HNO, CuDHX1 displays a five-fold fluorescence turn-on in cuvettes and is selective for HNO over thiols and reactive nitrogen and oxygen species. CuDHX1 can detect exogenously applied HNO in live mammalian cells and in conjunction with the zinc-specific, green-fluorescent sensor ZP1 can perform multicolor/multianalyte microscopic imaging. These studies reveal that HNO treatment elicits an increase in the concentration of intracellular mobile zinc.