Insights into the Physiology, Evolution, and Design of Bacterial Chlorate Reduction
- Clark, Iain Christopher
- Advisor(s): Hermanowicz, Slawomir W
Abstract
Bacterial chlorate respiration is an important part of the biogeochemical cycling of chlorine in the environment, but very little is known about the metabolism at the molecular level. This work extends our current understanding of microbial chlorate reduction by genome sequencing isolates and uncovering the novel architecture of chlorate reduction composite transposons (Chapter 2); by characterizing the physiology of an environmental isolate Shewanella algae ACDC using proteomics, RNA-seq, and genetics (Chapter 3); by engineering the ability to reduce chlorate de novo in the non-chlorate reducer Shewanella oneidensis MR-1 (Chapter 4); and by applying genetic tools (Bar-seq, allelic exchange) to dissect the metabolism in Pseudomonas stutzeri PDA (Chapter 5).