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Multimodal microtubule binding by the Ndc80 kinetochore complex.

  • Author(s): Alushin, Gregory
  • Musinipally, Vivek
  • Matson, Daniel
  • Tooley, John
  • Stukenberg, P
  • NOGALES, Eva
  • et al.

Published Web Location

https://doi.org/10.1038/nsmb.2411
Abstract

The Ndc80 complex is a key site of kinetochore-microtubule attachment during cell division. The human complex engages microtubules with a globular head formed by tandem calponin-homology domains and an 80-amino-acid unstructured tail that contains sites of phosphoregulation by the Aurora B kinase. Using biochemical, cell biological and electron microscopy analyses, we dissected the roles of the tail in binding of microtubules and mediation of cooperative interactions between Ndc80 complexes. Two segments of the tail that contain Aurora B phosphorylation sites become ordered at interfaces; one with tubulin and the second with an adjacent Ndc80 head on the microtubule surface, forming interactions that are disrupted by phosphorylation. We propose a model in which Ndc80s interaction with either growing or shrinking microtubule ends can be tuned by the phosphorylation state of its tail.

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