Skip to main content
eScholarship
Open Access Publications from the University of California
Refine Search

UC Irvine

UC Irvine Previously Published Works bannerUC Irvine

Three-dimensional early retinal progenitor 3D tissue constructs derived from human embryonic stem cells

Published Web Location

https://doi.org/10.1016/j.jneumeth.2010.04.025
Abstract

Purpose

To develop three-dimensional (3D) constructs of retinal pigment epithelium (RPE) and early retina progenitor cells from human embryonic stem cells (hESCs).

Methods

3D tissue constructs were developed by culturing hESC-derived neural retinal progenitors in a matrix on top of hESC-derived RPE cells in a cell culture insert. An osmolarity gradient maintained the nutrition of the 3D cell constructs. Cross-sections through hESC-derived tissue constructs were characterized by immunohistochemistry for various transcription factors and cell markers.

Results

hESC-derived tissue constructs expressed transcription factors characteristic of retinal development, such as pax6, Otx2, Chx10, retinal RAX; Brn3b (necessary for differentiation of retinal ganglion cells); and crx and nrl (role in photoreceptor development). Many cells expressed neuronal markers including nestin, beta-tubulin and microtubule-associated proteins.

Conclusions

This study shows for the first time that 3D early retinal progenitor tissue constructs can be derived from hESCs.

Many UC-authored scholarly publications are freely available on this site because of the UC's open access policies. Let us know how this access is important for you.

Main Content
For improved accessibility of PDF content, download the file to your device.
Current View


Top