Lawrence Berkeley National Laboratory
Applications of CRISPR-Cas Enzymes in Cancer Therapeutics and Detection.
- Author(s): Huang, Chun-Hao
- Lee, Ko-Chuan
- Doudna, Jennifer A
- et al.
Published Web Locationhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6299457/
Cancer is a complex disease caused by combinations of cellular genetic alterations and heterogeneous microenvironments. The use of the robust and programmable CRISPR-Cas systems has greatly improved genome editing for precision cancer modeling and enabled multiplexed genetic manipulation for cancer treatment and mutation detection. In this review, we outline the current CRISPR-Cas toolkit, and discuss the promises and hurdles in translating this revolutionary technology into effective and safe clinical applications for cancer treatment and diagnosis.