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Comparative genomics of Cluster O mycobacteriophages.

  • Author(s): Cresawn, Steven G
  • Pope, Welkin H
  • Jacobs-Sera, Deborah
  • Bowman, Charles A
  • Russell, Daniel A
  • Dedrick, Rebekah M
  • Adair, Tamarah
  • Anders, Kirk R
  • Ball, Sarah
  • Bollivar, David
  • Breitenberger, Caroline
  • Burnett, Sandra H
  • Butela, Kristen
  • Byrnes, Deanna
  • Carzo, Sarah
  • Cornely, Kathleen A
  • Cross, Trevor
  • Daniels, Richard L
  • Dunbar, David
  • Findley, Ann M
  • Gissendanner, Chris R
  • Golebiewska, Urszula P
  • Hartzog, Grant A
  • Hatherill, J Robert
  • Hughes, Lee E
  • Jalloh, Chernoh S
  • De Los Santos, Carla
  • Ekanem, Kevin
  • Khambule, Sphindile L
  • King, Rodney A
  • King-Smith, Christina
  • Klyczek, Karen
  • Krukonis, Greg P
  • Laing, Christian
  • Lapin, Jonathan S
  • Lopez, A Javier
  • Mkhwanazi, Sipho M
  • Molloy, Sally D
  • Moran, Deborah
  • Munsamy, Vanisha
  • Pacey, Eddie
  • Plymale, Ruth
  • Poxleitner, Marianne
  • Reyna, Nathan
  • Schildbach, Joel F
  • Stukey, Joseph
  • Taylor, Sarah E
  • Ware, Vassie C
  • Wellmann, Amanda L
  • Westholm, Daniel
  • Wodarski, Donna
  • Zajko, Michelle
  • Zikalala, Thabiso S
  • Hendrix, Roger W
  • Hatfull, Graham F
  • et al.
Abstract

Mycobacteriophages--viruses of mycobacterial hosts--are genetically diverse but morphologically are all classified in the Caudovirales with double-stranded DNA and tails. We describe here a group of five closely related mycobacteriophages--Corndog, Catdawg, Dylan, Firecracker, and YungJamal--designated as Cluster O with long flexible tails but with unusual prolate capsids. Proteomic analysis of phage Corndog particles, Catdawg particles, and Corndog-infected cells confirms expression of half of the predicted gene products and indicates a non-canonical mechanism for translation of the Corndog tape measure protein. Bioinformatic analysis identifies 8-9 strongly predicted SigA promoters and all five Cluster O genomes contain more than 30 copies of a 17 bp repeat sequence with dyad symmetry located throughout the genomes. Comparison of the Cluster O phages provides insights into phage genome evolution including the processes of gene flux by horizontal genetic exchange.

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