Skip to main content
eScholarship
Open Access Publications from the University of California
Refine Search

UC San Diego

UC San Diego Previously Published Works bannerUC San Diego

Detection, Isolation, and Functional Studies of Mouse Pulmonary Group 2 Innate Lymphoid Cells

Published Web Location

https://doi.org/10.1007/978-1-0716-2364-0_12
Abstract

ILC2s are key players in the emergence of type 2 inflammation in many pulmonary diseases. While several phenotypic markers can be used to identify ILC2s, our method utilizes the surface markers CD127 and ST2 to classify a group of type 2 cytokine-producing ILC2s upon activation by the fungal allergen Alternaria alternata . Here, we provide our protocol for the detection and isolation of a highly pure population of pulmonary mouse ILCs via flow cytometry and cell sorting. We also describe the methods for in vitro stimulation to assess the functionality of ILC2s.

Many UC-authored scholarly publications are freely available on this site because of the UC's open access policies. Let us know how this access is important for you.

Main Content
For improved accessibility of PDF content, download the file to your device.
Current View


Top