A low-cost open-source SNP genotyping platform for association mapping applications.
- Author(s): Macdonald, Stuart J
- Pastinen, Tomi
- Genissel, Anne
- Cornforth, Theodore W
- Long, Anthony D
- et al.
Published Web Locationhttps://doi.org/10.1186/gb-2005-6-12-r105
Association mapping aimed at identifying DNA polymorphisms that contribute to variation in complex traits entails genotyping a large number of single-nucleotide polymorphisms (SNPs) in a very large panel of individuals. Few technologies, however, provide inexpensive high-throughput genotyping. Here, we present an efficient approach developed specifically for genotyping large fixed panels of diploid individuals. The cost-effective, open-source nature of our methodology may make it particularly attractive to those working in nonmodel systems.