Skip to main content
Open Access Publications from the University of California


UC San Francisco Previously Published Works bannerUCSF

Increased Hematopoietic Extracellular RNAs and Vesicles in the Lung during Allergic Airway Responses.

  • Author(s): Pua, Heather H;
  • Happ, Hannah C;
  • Gray, Carleigh J;
  • Mar, Darryl J;
  • Chiou, Ni-Ting;
  • Hesse, Laura E;
  • Ansel, K Mark
  • et al.

Extracellular RNAs (exRNAs) can be released by numerous cell types in vitro, are often protected within vesicles, and can modify recipient cell function. To determine how the composition and cellular sources of exRNAs and the extracellular vesicles (EVs) that carry them change in vivo during tissue inflammation, we analyzed bronchoalveolar lavage fluid (BALF) from mice before and after lung allergen challenge. In the lung, extracellular microRNAs (ex-miRNAs) had a composition that was highly correlated with airway-lining epithelium. Using cell type-specific membrane tagging and single vesicle flow, we also found that 80% of detected vesicles were of epithelial origin. After the induction of allergic airway inflammation, miRNAs selectively expressed by immune cells, including miR-223 and miR-142a, increased and hematopoietic-cell-derived EVs also increased >2-fold. These data demonstrate that infiltrating immune cells release ex-miRNAs and EVs in inflamed tissues to alter the local extracellular environment.

Many UC-authored scholarly publications are freely available on this site because of the UC's open access policies. Let us know how this access is important for you.

Main Content
For improved accessibility of PDF content, download the file to your device.
Current View