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A turn-off fluorescent substrate for horseradish peroxidase improves the sensitivity of ELISAs

  • Author(s): Acharya, AP;
  • Sen, P;
  • Aran, K;
  • Gardner, AB;
  • Rafi, M;
  • Dean, D;
  • Murthy, N
  • et al.

Published Web Location

https://doi.org/10.1002/pola.27376
Abstract

A new turn-off fluorescent substrate for horseradish peroxidase is introduced, termed clics, which enhances the sensitivity of commercial enzyme-linked immunosorbant assays (ELISA) by 32-fold and detects chlamydial infections in patients. Clics are cost-effective, stable, and can be easily incorporated in commercial ELISA kits, and therefore should have a great impact in the fields of medicine and biology.

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