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Quality-filtering vastly improves diversity estimates from Illumina amplicon sequencing
- Bokulich, Nicholas A;
- Subramanian, Sathish;
- Faith, Jeremiah J;
- Gevers, Dirk;
- Gordon, Jeffrey I;
- Knight, Rob;
- Mills, David A;
- Caporaso, J Gregory
Published Web Location
https://doi.org/10.1038/nmeth.2276Abstract
A systematic evaluation of parameters that affect short amplicons sequenced on Illumina platforms provides guidelines for how to eliminate erroneous reads and improve data interpretation.
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