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CD13 and ROR2 Permit Isolation of Highly Enriched Cardiac Mesoderm from Differentiating Human Embryonic Stem Cells.

  • Author(s): Skelton, Rhys JP
  • Brady, Bevin
  • Khoja, Suhail
  • Sahoo, Debashis
  • Engel, James
  • Arasaratnam, Deevina
  • Saleh, Kholoud K
  • Abilez, Oscar J
  • Zhao, Peng
  • Stanley, Edouard G
  • Elefanty, Andrew G
  • Kwon, Murray
  • Elliott, David A
  • Ardehali, Reza
  • et al.

Published Web Location

http://www.ncbi.nlm.nih.gov/pubmed/26771355
No data is associated with this publication.
Abstract

The generation of tissue-specific cell types from human embryonic stem cells (hESCs) is critical for the development of future stem cell-based regenerative therapies. Here, we identify CD13 and ROR2 as cell-surface markers capable of selecting early cardiac mesoderm emerging during hESC differentiation. We demonstrate that the CD13+/ROR2+ population encompasses pre-cardiac mesoderm, which efficiently differentiates to all major cardiovascular lineages. We determined the engraftment potential of CD13+/ROR2+ in small (murine) and large (porcine) animal models, and demonstrated that CD13+/ROR2+ progenitors have the capacity to differentiate toward cardiomyocytes, fibroblasts, smooth muscle, and endothelial cells in vivo. Collectively, our data show that CD13 and ROR2 identify a cardiac lineage precursor pool that is capable of successful engraftment into the porcine heart. These markers represent valuable tools for further dissection of early human cardiac differentiation, and will enable a detailed assessment of human pluripotent stem cell-derived cardiac lineage cells for potential clinical applications.

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