Skip to main content
eScholarship
Open Access Publications from the University of California

Characterization of two crystal forms of cytochrome c from Valida membranaefaciens.

  • Author(s): Day, J
  • McPherson, A
  • et al.
Abstract

Two different crystal forms of cytochrome c from the yeast Valida membranaefaciens have been grown by vapor diffusion from polyethylene glycol 4000. Both have been characterized by X-ray diffraction. The first crystal form was grown at 277 K but proved unstable and dissolved when the temperature was increased above 288 K. This crystal was of triclinic space group P1 and had unit-cell dimensions of a = 68.4, b = 35.7, c = 33.24 A, alpha = 116.9, beta = 114.9, gamma = 75.0 degrees. Following dissolution of the triclinic crystals, the second crystal form also grew at 277 K but remained stable even at room temperature. It was of orthorhombic space group P2(1)2(1)2(1) with cell dimensions a = 27.95, b = 63.68, and c = 64.28 A. Although rather small in size, both crystal forms diffracted X-rays well and the resolution of the patterns extended beyond 3 A.

Many UC-authored scholarly publications are freely available on this site because of the UC Academic Senate's Open Access Policy. Let us know how this access is important for you.

Main Content
Current View