Elevated levels of G-quadruplex formation in human stomach and liver cancer tissues.
- Author(s): Biffi, Giulia;
- Tannahill, David;
- Miller, Jodi;
- Howat, William J;
- Balasubramanian, Shankar
- et al.
Published Web Locationhttps://doi.org/10.1371/journal.pone.0102711
Four-stranded G-quadruplex DNA secondary structures have recently been visualized in the nuclei of human cultured cells. Here, we show that BG4, a G-quadruplex-specific antibody, can be used to stain DNA G-quadruplex structures in patient-derived tissues using immunohistochemistry. We observe a significantly elevated number of G-quadruplex-positive nuclei in human cancers of the liver and stomach as compared to background non-neoplastic tissue. Our results suggest that G-quadruplex formation can be detected and measured in patient-derived material and that elevated G-quadruplex formation may be a characteristic of some cancers.