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jYCaMP: an optimized calcium indicator for two-photon imaging at fiber laser wavelengths
- Mohr, Manuel Alexander;
- Bushey, Daniel;
- Aggarwal, Abhi;
- Marvin, Jonathan S;
- Kim, Jeong Jun;
- Marquez, Emiliano Jimenez;
- Liang, Yajie;
- Patel, Ronak;
- Macklin, John J;
- Lee, Chi-Yu;
- Tsang, Arthur;
- Tsegaye, Getahun;
- Ahrens, Allison M;
- Chen, Jerry L;
- Kim, Douglas S;
- Wong, Allan M;
- Looger, Loren L;
- Schreiter, Eric R;
- Podgorski, Kaspar
- et al.
Published Web Location
https://doi.org/10.1038/s41592-020-0835-7Abstract
Femtosecond lasers at fixed wavelengths above 1,000 nm are powerful, stable and inexpensive, making them promising sources for two-photon microscopy. Biosensors optimized for these wavelengths are needed for both next-generation microscopes and affordable turn-key systems. Here we report jYCaMP1, a yellow variant of the calcium indicator jGCaMP7 that outperforms its parent in mice and flies at excitation wavelengths above 1,000 nm and enables improved two-color calcium imaging with red fluorescent protein-based indicators.
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