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PrimerProspector: de novo design and taxonomic analysis of barcoded polymerase chain reaction primers.

  • Author(s): Walters, William A;
  • Caporaso, J Gregory;
  • Lauber, Christian L;
  • Berg-Lyons, Donna;
  • Fierer, Noah;
  • Knight, Rob
  • et al.
Abstract

Motivation

PCR amplification of DNA is a key preliminary step in many applications of high-throughput sequencing technologies, yet design of novel barcoded primers and taxonomic analysis of novel or existing primers remains a challenging task.

Results

PrimerProspector is an open-source software package that allows researchers to develop new primers from collections of sequences and to evaluate existing primers in the context of taxonomic data.

Availability

PrimerProspector is open-source software available at http://pprospector.sourceforge.net

Contact

rob.knight@colorado.edu

Supplementary information

Supplementary data are available at Bioinformatics online.

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