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PrimerProspector: De novo design and taxonomic analysis of barcoded polymerase chain reaction primers

  • Author(s): Walters, WA
  • Caporaso, JG
  • Lauber, CL
  • Berg-Lyons, D
  • Fierer, N
  • Knight, R
  • et al.
Abstract

Motivation: PCR amplification of DNA is a key preliminary step in many applications of high-throughput sequencing technologies, yet design of novel barcoded primers and taxonomic analysis of novel or existing primers remains a challenging task. Results: PrimerProspector is an open-source software package that allows researchers to develop new primers from collections of sequences and to evaluate existing primers in the context of taxonomic data. © The Author(s) 2011. Published by Oxford University Press.

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