UC San Diego
PrimerProspector: de novo design and taxonomic analysis of barcoded polymerase chain reaction primers.
- Author(s): Walters, William A
- Caporaso, J Gregory
- Lauber, Christian L
- Berg-Lyons, Donna
- Fierer, Noah
- Knight, Rob
- et al.
Published Web Locationhttps://doi.org/10.1093/bioinformatics/btr087
PCR amplification of DNA is a key preliminary step in many applications of high-throughput sequencing technologies, yet design of novel barcoded primers and taxonomic analysis of novel or existing primers remains a challenging task.PrimerProspector is an open-source software package that allows researchers to develop new primers from collections of sequences and to evaluate existing primers in the context of taxonomic data.PrimerProspector is open-source software available at http://firstname.lastname@example.orgSupplementary data are available at Bioinformatics online.