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PrimerProspector: de novo design and taxonomic analysis of barcoded polymerase chain reaction primers.
- Author(s): Walters, William A;
- Caporaso, J Gregory;
- Lauber, Christian L;
- Berg-Lyons, Donna;
- Fierer, Noah;
- Knight, Rob
- et al.
Published Web Locationhttps://doi.org/10.1093/bioinformatics/btr087
MotivationPCR amplification of DNA is a key preliminary step in many applications of high-throughput sequencing technologies, yet design of novel barcoded primers and taxonomic analysis of novel or existing primers remains a challenging task.
ResultsPrimerProspector is an open-source software package that allows researchers to develop new primers from collections of sequences and to evaluate existing primers in the context of taxonomic data.
AvailabilityPrimerProspector is open-source software available at http://pprospector.sourceforge.net
Supplementary informationSupplementary data are available at Bioinformatics online.
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