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2ab assembly: a methodology for automatable, high-throughput assembly of standard biological parts

  • Author(s): Leguia, Mariana
  • Brophy, Jennifer AN
  • Densmore, Douglas
  • Asante, Angel
  • Anderson, J Christopher
  • et al.
Abstract

Abstract There is growing demand for robust DNA assembly strategies to quickly and accurately fabricate genetic circuits for synthetic biology. One application of this technology is reconstitution of multi-gene assemblies. Here, we integrate a new software tool chain with 2ab assembly and show that it is robust enough to generate 528 distinct composite parts with an error-free success rate of 96%. Finally, we discuss our findings in the context of its implications for biosafety and biosecurity.

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