- Main
Ensembles of endothelial and mural cells promote angiogenesis in prenatal human brain
- Crouch, Elizabeth E;
- Bhaduri, Aparna;
- Andrews, Madeline G;
- Cebrian-Silla, Arantxa;
- Diafos, Loukas N;
- Birrueta, Janeth Ochoa;
- Wedderburn-Pugh, Kaylee;
- Valenzuela, Edward J;
- Bennett, Neal K;
- Eze, Ugomma C;
- Sandoval-Espinosa, Carmen;
- Chen, Jiapei;
- Mora, Cristina;
- Ross, Jayden M;
- Howard, Clare E;
- Gonzalez-Granero, Susana;
- Lozano, Jaime Ferrer;
- Vento, Maximo;
- Haeussler, Maximilian;
- Paredes, Mercedes F;
- Nakamura, Ken;
- Garcia-Verdugo, Jose Manuel;
- Alvarez-Buylla, Arturo;
- Kriegstein, Arnold R;
- Huang, Eric J
- et al.
Published Web Location
https://doi.org/10.1016/j.cell.2022.09.004Abstract
Interactions between angiogenesis and neurogenesis regulate embryonic brain development. However, a comprehensive understanding of the stages of vascular cell maturation is lacking, especially in the prenatal human brain. Using fluorescence-activated cell sorting, single-cell transcriptomics, and histological and ultrastructural analyses, we show that an ensemble of endothelial and mural cell subtypes tile the brain vasculature during the second trimester. These vascular cells follow distinct developmental trajectories and utilize diverse signaling mechanisms, including collagen, laminin, and midkine, to facilitate cell-cell communication and maturation. Interestingly, our results reveal that tip cells, a subtype of endothelial cells, are highly enriched near the ventricular zone, the site of active neurogenesis. Consistent with these observations, prenatal vascular cells transplanted into cortical organoids exhibit restricted lineage potential that favors tip cells, promotes neurogenesis, and reduces cellular stress. Together, our results uncover important mechanisms into vascular maturation during this critical period of human brain development.
Many UC-authored scholarly publications are freely available on this site because of the UC's open access policies. Let us know how this access is important for you.
Main Content
Enter the password to open this PDF file:
-
-
-
-
-
-
-
-
-
-
-
-
-
-