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A synthetic luciferin improves bioluminescence imaging in live mice
- Evans, Melanie S;
- Chaurette, Joanna P;
- Adams, Spencer T;
- Reddy, Gadarla R;
- Paley, Miranda A;
- Aronin, Neil;
- Prescher, Jennifer A;
- Miller, Stephen C
Published Web Location
https://doi.org/10.1038/nmeth.2839Abstract
Firefly luciferase is the most widely used optical reporter for noninvasive bioluminescence imaging (BLI) in rodents. BLI relies on the ability of the injected luciferase substrate D-luciferin to access luciferase-expressing cells and tissues within the animal. Here we show that injection of mice with a synthetic luciferin, CycLuc1, improves BLI with existing luciferase reporters and enables imaging in the brain that could not be achieved with D-luciferin.
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