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A synthetic luciferin improves bioluminescence imaging in live mice

  • Author(s): Evans, MS
  • Chaurette, JP
  • Adams, ST
  • Reddy, GR
  • Paley, MA
  • Aronin, N
  • Prescher, JA
  • Miller, SC
  • et al.
Abstract

Firefly luciferase is the most widely used optical reporter for noninvasive bioluminescence imaging (BLI) in rodents. BLI relies on the ability of the injected luciferase substrate D-luciferin to access luciferase-expressing cells and tissues within the animal. Here we show that injection of mice with a synthetic luciferin, CycLuc1, improves BLI with existing luciferase reporters and enables imaging in the brain that could not be achieved with D-luciferin. © 2014 Nature America, Inc. All rights reserved.

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