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A synthetic luciferin improves bioluminescence imaging in live mice.

  • Author(s): Evans, Melanie S
  • Chaurette, Joanna P
  • Adams, Spencer T
  • Reddy, Gadarla R
  • Paley, Miranda A
  • Aronin, Neil
  • Prescher, Jennifer A
  • Miller, Stephen C
  • et al.
Abstract

Firefly luciferase is the most widely used optical reporter for noninvasive bioluminescence imaging (BLI) in rodents. BLI relies on the ability of the injected luciferase substrate D-luciferin to access luciferase-expressing cells and tissues within the animal. Here we show that injection of mice with a synthetic luciferin, CycLuc1, improves BLI with existing luciferase reporters and enables imaging in the brain that could not be achieved with D-luciferin.

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