Skip to main content
Open Access Publications from the University of California

In vitro cytotoxicity of human recombinant tumor necrosis factor α in association with radiotherapy in a human ovarian carcinoma cell line

  • Author(s): Manetta, A
  • Lucci, J
  • Soopikian, J
  • Granger, G
  • Berman, ML
  • DiSaia, PJ
  • et al.

It has been speculated that tumor necrosis factor α (TNF-α) may decrease the cytotoxicity of radiotherapy by increasing the scavenging of toxic superoxide radicals. Because of the possible clinical implications, the cytotoxicity of TNF-α in combination with radiotherapy (RT) was compared with that of RT alone in a human ovarian cancer cell line. NIH:OVCAR-3 cells were incubated with TNF-α at 10.0, 1.0, 0.1, and 0.01 μg/ml. Plates were divided into two groups; one received 150 cGy of radio-therapy and the other received no further therapy. Seventy-two hours later, supernatants were aspirated and viable cells were stained with a 1% solution of crystal violet. Survival of cells treated with RT plus TNF-α was expressed as a percentage of surviving irradiated controls. Analysis of results revealed minimal additive cell killing effect between TNF-α and radiotherapy at all concentrations of tumor necrosis factor, with the greatest difference noted in the group treated with 10 μg/ml TNF-α. A continued radiotherapy dose-response study with TNF-α showed a similar additive, not radioprotective, effect. This may have implication as a potentiator of RT in some human tumors. © 1990.

Many UC-authored scholarly publications are freely available on this site because of the UC Academic Senate's Open Access Policy. Let us know how this access is important for you.

Main Content
Current View