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Coherent movement of cell layers during wound healing by image correlation spectroscopy

  • Author(s): Tanner, K
  • Ferris, DR
  • Lanzano, L
  • Mandefro, B
  • Mantulin, WW
  • Gardiner, DM
  • Rugg, EL
  • Gratton, E
  • et al.
Abstract

We have determined the complex sequence of events from the point of injury until reepithelialization in axolotl skin explant model and shown that cell layers move coherently driven by cell swelling after injury. We quantified three-dimensional cell migration using correlation spectroscopy and resolved complex dynamics such as the formation of dislocation points and concerted cell motion. We quantified relative behavior such as velocities and swelling of cells as a function of cell layer during healing. We propose that increased cell volume (∼37% at the basal layer) is the driving impetus for the start of cell migration after injury where the enlarged cells produce a point of dislocation that foreshadows and dictates the initial direction of the migrating cells. Globally, the cells follow a concerted vortex motion that is maintained after wound closure. Our results suggest that cell volume changes the migration of the cells after injury. © 2009 by the Biophysical Society.

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