Skip to main content
eScholarship
Open Access Publications from the University of California

Neutral Zone Classifiers Using a Decision-Theoretic Approach With Application to DNA Array Analyses

  • Author(s): Yu, Hua
  • Jeske, Daniel R.
  • Ruegger, Paul
  • Borneman, James
  • et al.
Abstract

Two-class neutral zone classifiers were recently proposed for use in microbial community profiling applications. These classifiers allow a region of neutrality for cases where probe hybridization outcomes are too ambiguous to have adequate confidence in assigning a “binding” or “no binding” result. In this paper, we generalize the idea of neutral zone classifiers to an arbitrary number of classes and apply it to improve the process of microbial community profiling by considering a third class for the outcome of probe hybridization experiments, “partial binding.” We introduce a family of class distributions that uses a mixture of Gaussian distributions as a model for a Box–Cox power transformation of the raw intensity measurements. Stratified cross-validation analyses are used to assess the efficacy of the proposed three-class neutral zone classifier. This article has supplementary material online.

Many UC-authored scholarly publications are freely available on this site because of the UC Academic Senate's Open Access Policy. Let us know how this access is important for you.

Main Content
Current View