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Genome-wide profiling of human cap-independent translation-enhancing elements.

  • Author(s): Wellensiek, Brian P
  • Larsen, Andrew C
  • Stephens, Bret
  • Kukurba, Kim
  • Waern, Karl
  • Briones, Natalia
  • Liu, Li
  • Snyder, Michael
  • Jacobs, Bertram L
  • Kumar, Sudhir
  • Chaput, John C
  • et al.

We report an in vitro selection strategy to identify RNA sequences that mediate cap-independent initiation of translation. This method entails mRNA display of trillions of genomic fragments, selection for initiation of translation and high-throughput deep sequencing. We identified >12,000 translation-enhancing elements (TEEs) in the human genome, generated a high-resolution map of human TEE-bearing regions (TBRs), and validated the function of a subset of sequences in vitro and in cultured cells.

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