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Open Access Publications from the University of California

Reliability of human cortical organoid generation.

  • Author(s): Yoon, Se-Jin
  • Elahi, Lubayna S
  • Pașca, Anca M
  • Marton, Rebecca M
  • Gordon, Aaron
  • Revah, Omer
  • Miura, Yuki
  • Walczak, Elisabeth M
  • Holdgate, Gwendolyn M
  • Fan, H Christina
  • Huguenard, John R
  • Geschwind, Daniel H
  • Pașca, Sergiu P
  • et al.

The differentiation of pluripotent stem cells in three-dimensional cultures can recapitulate key aspects of brain development, but protocols are prone to variable results. Here we differentiated multiple human pluripotent stem cell lines for over 100 d using our previously developed approach to generate brain-region-specific organoids called cortical spheroids and, using several assays, found that spheroid generation was highly reliable and consistent. We anticipate the use of this approach for large-scale differentiation experiments and disease modeling.

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