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Sensitivity of RT-PCR method in samples shown to be positive for Zika virus by RT-qPCR in vector competence studies
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https://doi.org/10.1590/1678-4685-gmb-2016-0312Abstract
Tissue samples from mosquitoes artificially infected with Zika virus and shown to be positive by RT-qPCR were reexamined by RT-PCR. Using these samples we compared the two methods employed in virus RNA detection for vector competence studies. Results demonstrated that, albeit useful, RT-PCR gave false negatives with low viral loads (< 106 RNA copies/ml).
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