Skip to main content
eScholarship
Open Access Publications from the University of California

An improved In Vitro assay for Lymphotoxin

  • Author(s): Kramer, JJ
  • Granger, GA
  • et al.
Abstract

An In Vitro Microassay for Lymphotoxin (LT) is described. Target cell monolayers are established in the wells of a microtiter assay plate at a density of 100-500/well. Dilutions of Lymphotoxin containing medium are placed upon the target cell monolayers. The plates are sealed with a gas impervious film, and the cells are incubated for 24-48 hr. The cell numbers in each well are established initially, and after the incubation period. The percent destruction is based upon total cell counts of experimental wells compared to control wells. Comparison of the assay to the previously described assay, which is based upon ability of cells to incorporate14C amino acids into protein, with several batches of LT, shows the microassay to be 25 × more sensitive than the latter. Other advantages and disadvantages of the assay system are also described. © 1972.

Many UC-authored scholarly publications are freely available on this site because of the UC Academic Senate's Open Access Policy. Let us know how this access is important for you.

Main Content
Current View