Unilateral hypoxic brain perfusion for generation of cerebral ischemic injury in rat.
Unilateral hypoxic ischemia via common carotid artery infusion was not achieved. Despite evidence of hippocampal necrosis as indicated by Fluoro-Jade immunohistochemistry, animals displayed no qualitative neurological deficits. It is likely that intra-ischemic systemic and intracranial hypothermia created a neuroprotective environment during infusion. Further studies will necessitate sufficient temperature control based on intracranial temperatures.