A Unique Sulfotransferase-Involving Strigolactone Biosynthetic Route in Sorghum.
Published Web Locationhttps://www.frontiersin.org/articles/10.3389/fpls.2021.793459/full#:~:text=A%20Unique%20Sulfotransferase-Involving%20Strigolactone%20Biosynthetic%20Route%20in%20Sorghum,-Sheng%20Wu%20and&text=LOW%20GERMINATION%20STIMULANT%201%20(LGS1,the%20catalytic%20function%20remains%20unclear.
LOW GERMINATION STIMULANT 1 (LGS1) plays an important role in strigolactones (SLs) biosynthesis and Striga resistance in sorghum, but the catalytic function remains unclear. Using the recently developed SL-producing microbial consortia, we examined the activities of sorghum MORE AXILLARY GROWTH1 (MAX1) analogs and LGS1. Surprisingly, SbMAX1a (cytochrome P450 711A enzyme in sorghum) synthesized 18-hydroxy-carlactonoic acid (18-hydroxy-CLA) directly from carlactone (CL) through four-step oxidations. The further oxidated product orobanchol (OB) was also detected in the microbial consortium. Further addition of LGS1 led to the synthesis of both 5-deoxystrigol (5DS) and 4-deoxyorobanchol (4DO). Further biochemical characterization found that LGS1 functions after SbMAX1a by converting 18-hydroxy-CLA to 5DS and 4DO possibly through a sulfonation-mediated pathway. The unique functions of SbMAX1 and LGS1 imply a previously unknown synthetic route toward SLs.
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