Preparation of Urogenital Sinus Mesenchymal Cells for Prostate Tissue Recombination Models.
- Author(s): Zong, Yang
- Goldstein, Andrew S
- Witte, Owen N
- et al.
Published Web Locationhttps://doi.org/10.1101/pdb.prot078055
An appropriate microenvironment provided by the mesenchyme is important for establishing tissue recombination models for epithelial cancer. Urogenital sinus mesenchymal (UGSM) cells derived from embryonic rodent show potent inductive effects for prostate regeneration. Genetic manipulation of these mesenchymal cells allows us to define the contribution of the tumor microenvironment to prostate cancer development. This protocol describes preparation and propagation of murine UGSM cells in culture.