Validation of a diagnostic microarray for human papillomavirus: coverage of 102 genotypes.
- Author(s): Arron, Sarah Tuttleton
- Skewes-Cox, Peter
- Do, Phong H
- Dybbro, Eric
- Da Costa, Maria
- Palefsky, Joel M
- Derisi, Joseph L
- et al.
Published Web Locationhttps://doi.org/10.4061/2011/756905
Papillomaviruses have been implicated in a variety of human diseases ranging from common warts to invasive carcinoma of the anogenital mucosa. Existing assays for genotyping human papillomavirus are restricted to a small number of types. Here, we present a comprehensive, accurate microarray strategy for detection and genotyping of 102 human papillomavirus types and validate its use in a panel of 91 anal swabs. This array has equal performance to traditional dot blot analysis with the benefits of added genotype coverage and the ability to calibrate readout over a range of sensitivity or specificity values.