Entropy-regularized deconvolution of cellular cryotransmission electron tomograms.
- Author(s): Croxford, Matthew;
- Elbaum, Michael;
- Arigovindan, Muthuvel;
- Kam, Zvi;
- Agard, David;
- Villa, Elizabeth;
- Sedat, John
- et al.
Published Web Locationhttps://doi.org/10.1073/pnas.2108738118
Cryo-electron tomography (cryo-ET) allows for the high-resolution visualization of biological macromolecules. However, the technique is limited by a low signal-to-noise ratio (SNR) and variance in contrast at different frequencies, as well as reduced Z resolution. Here, we applied entropy-regularized deconvolution (ER-DC) to cryo-ET data generated from transmission electron microscopy (TEM) and reconstructed using weighted back projection (WBP). We applied deconvolution to several in situ cryo-ET datasets and assessed the results by Fourier analysis and subtomogram analysis (STA).