Lawrence Berkeley National Laboratory

Isoprene is highly-valued terpene based-chemical feedstock and can be derived from either petroleum or from fermentation of plant biomass. This project enabled more efficient isoprene fermentation using renewable resources and at yields that can compete economically with non-renewable sources. This Phase I project applied a novel synthetic biology approach, the Artificial Positive Feedback Loop (APFL) technology, to improve production yields of isoprene.