UC Riverside

Previous methods to determine equilibrium binding constants of proteins interacting with

ligands include isothermal titration calorimetry, surface plasma resonance and

fluorescence polarization. In this work, diffusion coefficients determined through Taylor

dispersion analysis are used to obtain equilibrium binding constants and complex

diffusion coefficients of ligands interacting with proteins. An equation developed by

Jensen et al 2010 is used to fit a plot of measured diffusion coefficients versus ligand

concentration. The best fit of this equation provides the equilibrium binding constant and

the diffusion coefficient of the ligand interacting with the protein.